Ipertermia e cellule di tumore della mammella
La rivista Oncology Report ha pubblicato nel Giugno 2011 uno studio in vitro sugli effetti dell'ipertermia sulle cellule MCF-7 di tumore mammario. Le risultanze sono state di una inibizione dell'attività di invasione delle suddette cellule nonché una soppressione dell'attività angiogenetica.
Affiliated Tumor Hospital of Guangzhou Medical College, Guangdong 510095, PR China.
The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-β1 expression. MCF-7 cells were heated at 43, 45, 47 and 37 °C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-β1 mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-β1, EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.
Oncol Rep. 2011 Jun;25(6):1573-9. doi: 10.3892/or.2011.1240. Epub 2011 Mar 29.